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. 2000 Aug 29;97(19):10555–10560. doi: 10.1073/pnas.180313097

Figure 5.

Figure 5

RT-PCR analysis of mRNA of GHRH receptor and its splice variants in human pituitary adenoma and CAKI-1 human RCC. Poly(A)+ RNA was reverse transcribed and amplified in PCR with primers for exon 3–4 (group 1), exon 7–8 (group 2), and intron 3–exon 8 (group 3) of human GHRH receptor gene. The secondary PCR products were separated electrophoretically on 1.5% agarose gels and stained with ethidium bromide. The PCR products were of the expected size of 144 bp (exon 3–4) (group 1); 147 bp (exon 7–8) (group 2); as well as 720 bp, 566 bp, and 335 bp (intron 3–exon 8) (group 3). Lanes: M, 100-bp DNA molecular weight marker; P, human pituitary adenoma; C, CAKI-1 RCC; −, RT-negative control from the mixture of poly(A)+ RNA from human pituitary adenoma and CAKI-1 RCC.