Fig. 1.
A flowchart illustrates the experimental procedures. The cells from the ilium were separated by a 70% Percoll™ (Amersham Pharmacia) gradient and the nucleated stroma cells were collected for primary cell cultures. Cells were seeded at a density of 1 × 105 cells/well. The cells reached first confluence 3 days later and second confluence 5 days after seeding. At the time of second confluence, gene expressions were analyzed. ALP activity and osteogenic and adipogenic differentiation were analyzed at the third, seventh, eleventh, and fourteenth days after the second confluence.