Fig. 7.

Light microscopic examinations of developing ocelli in Aurelia sp. 1. a, b Ephyra. c Metephyra. In a and c, rhopalia are labeled with antibodies against FMRFamide (FMRF) and acetylated tubulin (acTub). In c, phalloidin (Pha) is used to label filamentous actin. a, a′ Sections at the levels of the oral ectoderm, viewed from the oral side. In a, sections are taken from the apical cell surface (cilia not shown) of the oral ectoderm in the intermediate segment through the level of the internalized cilia of the developing cup-ocellus (co) at the base of the oral ectoderm. a′ is a single section at the base of the oral ectoderm in the developing cup-ocellus. Note the coiled cilia (ci) and surrounding dark space where endodermal pigment cells are located. b, b′ a bright-field image of the rhopalium, viewed from the oral side, showing accumulated pigment granules (pi) in the cup-ocellus (co). c A single section of the spot-ocellus (so) at the medial level, viewed from the lateral side. Lithocyst (lc) is to the left. Notice a cluster of FMRFamide-IR sensory cells (arrow) whose apical ends appear dark due to pigmentation (pi); basal neuronal processes (ne) are sent toward the oral-basal direction. lc lithocyst, o-d oral-distal group, o-c oral-central group, co pigment-cup ocellus, so pigment-spot ocellus, pi pigment granules, ci cilium, in intermediate segment, bs basal segment, ec ectoderm, en endoderm, ca rhopalar canal, ne neuronal processes