Figure 6.

Magnitude and Direction of the Linker Swing

In this figure, right and top views of the dynein head are related by a rotation about the y axis (see Figure S3 for details): each view has been rotated in the plane of the page relative to previous figures so that tag positions move only in the x direction (dashed lines).

(A) Tag positions in the unprimed motor are indicated by colored spots (as in Figure 4C).

(B) For the primed motor, the mean position of the N-terminal GFP tag (brighter magenta spots; derived for top view as explained in the main text) also moves only in the x direction. Magnitudes of the displacements from the unprimed positions (faded magenta spots) are indicated (arrows).

(C and D) Top and right views are related by a rotation of between 50° and 116° (see Figure S4 for details): illustrated here is a rotation of 90° (see also Movie S4). Tag positions in the unprimed conformation (C) and N-terminal GFP in the unprimed and primed conformations (D). In each case, the bottom surface of the cube shows their projected positions perpendicular to top and right views. The black arrow shows movement of N-terminal GFP during the priming stroke, which has a major component perpendicular to the plane of the top view and parallel to the plane of the right view. The 3D displacement of N-terminal GFP lies between 18.8 and 21.1 nm (depending on the rotation angle between right and top views).