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. Author manuscript; available in PMC: 2009 Jul 7.
Published in final edited form as: Exp Hematol. 2007 Apr;35(4 Suppl 1):78–86. doi: 10.1016/j.exphem.2007.01.028

Figure 5.

Figure 5

Titration of human (A) and murine (B) IL-31 with carboxyterminal His8-tag. Murine BaF3 cells transfected with hIL-31R/hOSM-Rβ and mIL-31R/mOSM-Rβ were incubated with increasing doses of human or murine IL-31. Proliferation was measured colorimetrically upon addition of XTT for the last 4 hours of the assay. Cross-species reactivity was not observed. EC50 for murine IL-31 was observed at 782 +/- 34 pg/ml, and EC50 for human IL-31 was 72 +/- 5 pg/ml.