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. Author manuscript; available in PMC: 2009 Jul 15.
Published in final edited form as: Anal Chem. 2008 May 24;80(14):5583–5589. doi: 10.1021/ac800549k

Figure 4. Separation results of a 1-kbp DNA ladder.

Figure 4

The sample contained 10 DNA fragments, and total DNA concentration was 50 ng/µL. The sample was injected at 100 psi for 10 s, and the separations were carried out at a chamber pressure of 100 psi. The separation traces were respectively obtained using an eluent containing (1) 50 µM TE buffer, (2) 100 µM TE buffer, (3) 10 mM TE buffer, (4) 100 mM TE buffer, and (5) 500 mM TE buffer. All other conditions were the same as indicated in Figure 2. (Note: The signals of traces 1 and 2 were multiplied by a factor of 0.25, and the signals of traces 3 and 4 were multiplied by a factor of 0.4 so that all five chromatograms could be arranged roughly evenly-spaced.)