All separation capillaries had the same length (46 cm total and 42 cm effective) but different radii, (A) 500 nm, (B) 800 nm and (C) 3000 nm, respectively. Sample injection conditions: (A) 10 s at 100 psi, (B) 5 s at 55 psi and (C) 4 s at 30 psi. The separation pressures were 100 psi, 55 psi and 30 psi respectively. Eluent was 10 mM TE (pH = 8.0).