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. Author manuscript; available in PMC: 2010 Apr 15.

Published in final edited form as: Anal Biochem. 2009 Feb 10;387(2):162–170. doi: 10.1016/j.ab.2009.01.028

a: The [¹²C₆]aniline and [¹³C₆]aniline derivatives of LNnT behave identically on normal phase HPLC analysis. An equimolar mixture of LNnT-[¹²C₆]aniline and LNnT-[¹³C₆]aniline co-chromatographed during normal phase separation by HPLC. The aniline derivatives were detected using UV absorption at 260 nm. A profile showing the chromatographic behavior of purified LNnT-[¹²C₆]aniline is inserted below as a reference. b: The 6 Da difference in [¹²C₆]aniline and [¹³C₆]aniline derivatives of LNnT are easily measured by ESI-MS analysis. The equimolar mixture of LNnT-[¹²C₆]aniline and LNnT-[¹³C₆]aniline was subjected to analysis by LC-MS. The structures are shown as symbols (Fig. 1) with the corresponding molecular masses.

a: The [¹²C₆]aniline and [¹³C₆]aniline derivatives of LNnT behave identically on normal phase HPLC analysis. An equimolar mixture of LNnT-[¹²C₆]aniline and LNnT-[¹³C₆]aniline co-chromatographed during normal phase separation by HPLC. The aniline derivatives were detected using UV absorption at 260 nm. A profile showing the chromatographic behavior of purified LNnT-[¹²C₆]aniline is inserted below as a reference. b: The 6 Da difference in [¹²C₆]aniline and [¹³C₆]aniline derivatives of LNnT are easily measured by ESI-MS analysis. The equimolar mixture of LNnT-[¹²C₆]aniline and LNnT-[¹³C₆]aniline was subjected to analysis by LC-MS. The structures are shown as symbols (Fig. 1) with the corresponding molecular masses.