Skip to main content
. Author manuscript; available in PMC: 2009 Jul 7.
Published in final edited form as: Biochemistry. 2008 May 14;47(24):6361–6369. doi: 10.1021/bi800657p

FIGURE 4. Inhibition and modification of wild-type SppA with FP-biotin.

FIGURE 4

Purified wild-type, S409A and SppA proteins were incubated in the presence or absence of FP-biotin (final 40 µM) for 10 min, followed by activity assays (A and C) or analyzed by SDS-polyacrylamide gel electrophoresis and immunoblotting with an avidin-horseradish peroxidase conjugate (B and D).