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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Immunogenetics. 2008 Oct 29;61(1):43–54. doi: 10.1007/s00251-008-0335-x

Figure 1.

Figure 1

The murine soluble tapasin mutant was not able to facilitate normal H2-Ld, -Kb, and -Kd surface expression. MF, MF+Ld, MF+Kb, or MF+Kd cells transfected with no tapasin, wild type mouse tapasin (Wt mTsn) or soluble mouse tapasin (Sol mTsn) were incubated with secondary antibody only, or with an antibody against the folded form of the transfected mouse MHC class I molecule (30-5-7 for Ld, Y3 for Kb, and 34-1-2 for Kd) or with 64-3-7 against the open form of each epitope-tagged mouse MHC class I molecule. Results obtained with the antibody against the folded form are shown in (A), and results obtained with 64-3-7 are shown in (B). Results obtained with secondary antibody only were all less than 5.0. Values on the y axes are relative mean fluorescence intensity (MFI) units. Very similar results were obtained with independent transfectant clones, with alternative antibodies (B8-24-3 for folded Kb), and in separate flow cytometry experiments.