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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Immunogenetics. 2008 Oct 29;61(1):43–54. doi: 10.1007/s00251-008-0335-x

Figure 4.

Figure 4

Soluble mouse tapasin partially restored B8 expression at the surface of 721.220 cells. (A) Cells (.220-B8, .220-B8+wt mTsn, and .220-B8+sol mTsn) were incubated with secondary antibody only (data not shown), or with an antibody against the folded form of the HLA class I molecule (W6/32), or with an antibody against the open form of the HLA class I molecule (HC10). Results obtained with the secondary antibody only were all less than 11.0. Values on the y axis are relative mean fluorescence intensity (MFI) units. Wt mTsn = wild type mouse tapasin; Sol mTsn = soluble mouse tapasin. Very similar results were obtained in a separate flow cytometry experiment. (B) Cells (.220-B8, .220-B8+wt mTsn, .220-B8+sol mTsn, .220-B8+wt hTsn, .220-B8+sol hTsn) were incubated with secondary antibody only, W6/32, or HC10. Results obtained with the secondary antibody only were all less than 7.0. The ratio of the mean fluorescence intensity for W6/32 versus HC10 for each cell type is displayed.