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. 2009 Jun 22;2:5. doi: 10.3389/neuro.02.005.2009

Figure 2.

Figure 2

Second generation voltage-sensitive fluorescent proteins. (A) Alignment of the amino acid sequences of VSFP2.1 variants. The C-terminal residues and downstream segment (240–254) of the VSD of Ci-VSP are shown in gray and italic text, respectively. FPs are mCerulean (blue), Citrine/mCitrine (yellow), mKate2 (red), mUKG (green) and mKOκ (orange). (B) Acceptor (upper color traces) and donor (lower color traces) signals of PC12 cells in response to a family of 500 ms voltage steps from a holding potential of −70 mV to test potentials of −140 to +60 mV recorded at 35°C. Traces corresponding to a depolarization to +60 mV are indicated by arrows (from Mutoh et al., 2009).