Figure 3.

Expression pattern of VSFP2.1 variants in transfected hippocampal neurons. Primary hippocampal cultures derived from mouse E18 embryos were transfected with either VSFP2.3, VSFP2.4 or Mermaid 6 days after plating and imaged by confocal fluorescence microscopy 1 week later. Fluorescence was allowed to saturate locally to optimize the visualization of neuronal processes. Boxed areas in the left panel are shown magnified on the right panel. Arrows indicate cell surface expression while arrowheads show intracellular expression. Note the targeting of VSFP2.3 and VSFP2.4 to the plasma membrane and Mermaid-associated intracellular aggregates in magnified views. The insert in the lower right image shows an example of a cell with clear expression of Mermaid at the cell surface. Scale bars are 50 and 10 μm for left and right panels, respectively.