Figure 3.

Smad8^lacZ expression pattern in postnatal tissues. (A and B) Smad8^lacZ expression was found in the prostate epithelium. (A) Wild-type control and (B) Smad8^lacZ −/− indicated that Smad8 was highly expressed in anterior and dorsal-lateral prostate lobes. (C and D) LacZ activity was detected in the stomach and the proximal duodenum. (C) Wild-type control and (D) Smad8^laZ heterozygous tissues. (E–G) LacZ activity was detected in whole mount lungs from P7 mice. (H–J) Sections of P7 lungs indicate that Smad8 was expressed in the lung parenchyma and in cells surrounding pulmonary vessels [outlined in (I)]. (J) is a section stained with H&E and LacZ. The arrow indicates a Smad8 expressing cells adjacent to a distal pulmonary artery. (K) is a wild-type embryo used as a negative control for LacZ activity. B, bladder; h, heart; i, intestine; s, stomach; p, prostate; sv, seminal vesicle.