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. 2009 May 9;18(15):2863–2874. doi: 10.1093/hmg/ddp221

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© The Author 2009. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

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Figure 5. — Co-immunoprecipitation assay of protein–protein interaction with MSX1. (Upper panels) Western blot analyses of whole cell lysates from COS7 cells cotransfected with Myc-tagged PAX9 proteins and FLAG-tagged MSX1. (Lower panels) Western blot analyses of proteins pulled down with α-FLAG antibody from whole cell lysates. Immunoprecipitated MSX1 was detected with the α-FLAG antibody and co-immunoprecipitated wild-type and missense mutant PAX9 proteins with antibody against the C-terminal transactivation domain of PAX9.