Figure 3.

Localization of demethylated cells in mutant brains by IAP immunostaining. (A) Western blotting of IAP protein in the dorsal cortex of wild type (con) and Emx1-cre; Dnmt1^2lox/2lox mutants mice (mut) and Nestin-cre; Dnmt1^2lox/2lox mutant mice (Nestin Dnmt1, see ref. 20). (B) Immunohistochemical detection of IAP in the dorsal cortex at 1 month of age in coronal sections. (scale bar = 100 microns) (C) Co-staining with NeuN (a pan-neuronal marker) reveals an IAP-positive staining pattern in mutant cortex only. (scale bar = 25 microns) (D) Cell counts show that approximately 28–32% of the total cells are IAP-positive in 1 or 2 months (mo) old mutant mice. Among those IAP+ cells, 70.67 ± 1.8% are NeuN+ neurons.