Fig. 3.

Southern blot analysis of process pools from a pilot lot of HA VRP. Lane 1, DNA size markers; lane 2, 1 ng of AluI-digested Vero DNA loaded on the gel as a control that underwent no sample processing; lane 3, salt wash harvest pool; lane 4, pool of VRP taken after Benzonase treatment; lane 5, pool of VRP taken after Benzonase treatment and spiked with 1 ng of AluI-digested Vero DNA; lane 6, pool of material at end of tangential flow filtration (TFF); lane 7, TFF pool spiked with 1 ng of AluI-digested Vero DNA; lane 8, Cellufine Sulfate elution pool; lane 9, Cellufine Sulfate elution pool spiked with 1 ng of AluI-digested Vero DNA. The locations of nucleotide size markers (kbp) are indicated on the left.