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. 2009 Jun 19;10:49. doi: 10.1186/1471-2121-10-49

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Copyright © 2009 Labriola et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cell spreading and migratory ability. (A) Cells from the different human insulin-producing cell lines were seeded and allowed to adhere for 60 min. After removal of non adherent cells, the percentage of spread-out cells was determined at 45 min. Data were collected by random observation and at least in triplicate. a p < 0.001. ANOVA/Bonferroni's test. (B) Confluent monolayers of the human cell lines and of normal islets were "wounded". The cells were allowed to migrate into the cell-free area for 18 h and then photographed. Cell migration was evaluated by densitometry, calculating the area occupied by the migratory cells. Migration is expressed as the percentage (mean ± SD) of wound covered area. a p < 0.001 normal islets vs cell lines. b p < 0.001 VGA vs APM and CPR cells. ANOVA/Bonferroni's test.