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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Protein Expr Purif. 2009 Mar 9;66(1):73–81. doi: 10.1016/j.pep.2009.03.001

Figure 2. Expression of chemokine receptors in different systems.

Figure 2

a) CCR1 variants containing different N- and/or C-terminal tags were stably transfected into mammalian cell systems (FlpIn/HEK293, T-Rex/HEK293, FlpIn/T-Rex/HEK293 and pACMV-TetO/HEK293). Where appropriate, cells were induced and cell surface receptor levels detected as described in the experimental procedures. b) Chemokine receptor variants CCR1, CCR2, CCR3 and CCR5 were transfected into HEK293 cells and expression levels of induced and un-induced cells were measured (n=4, shown as mean ± S.D.). Key: CCRXS: CCRX-StrepII, SCCRXH: StrepII-CCRX-His8, HACCRXH: Hemagglutinin-CCRX-His8.