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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Protein Expr Purif. 2009 Mar 9;66(1):73–81. doi: 10.1016/j.pep.2009.03.001

Figure 5. Purification of CCR1 in different lipid/detergent micelles.

Figure 5

a) Coomassie stained SDS-PAGE gel and b) western blot after solubilization and partial purification of CCR1 by Ni-affinity chromatography. The detergents/lipids used for solubilization were as follows: 1) 2% OG, 2) 0.2% DG, 3) 2% Cymal-5, 4) 1% Cymal-7, 5) 1% FC-14, 6) 2% Cyclofos-5, 7) 2% DM, 8) 1% DDM, 9) 1% TDM, 10) 1% DDM + 0.2% CHS, 11) 1% DDM + 0.02% CHS, 12) 2% OG + 0.02% CHS. Where possible, detergents were diluted 10-fold in column buffers after solubilization, but were always kept at concentrations >2-fold above the critical micelle concentration (CMC) values. C) Coomassie–stained gels of HA-His8 tagged (HAR1H) and StrepII-His8 tagged (SR1H) CCR1 after two-column purification.