Table 1.
Summary of the effects of different detergents upon the solubilization, oligomeric state and binding affinities of CCR1.
| Detergent/lipid system | Abbreviation | Properties of CCR1 in detergent system | ||
|---|---|---|---|---|
| Soluble? | Predominant oligomeric state* | KD for CCL14 binding | ||
| n-Octyl-β-D-glucopyranoside | OG | No | N.D. | |
| n-Decyl-β-D-glucopyranoside | DG | No | N.D. | |
| 5-Cyclohexyl-1-pentyl-β-D-maltoside | Cymal-5 | Yes | O | |
| 7-Cyclohexyl-1-heptyl-β-D-maltoside | Cymal-7 | Yes | O | |
| n-Dodecylphosphocholine | FC-12 | Yes | N.D. | >1uM |
| n-Tetradecylphosphocholine | FC-14 | Yes | M | >1uM |
| n-Hexadecylphosphocholine | FC-16 | Yes | N.D. | >1uM |
| 5-Cyclohexyl-1-2-pentylphosphocholine | Cyclofos-5 | Yes | O | |
| n-Decyl-β-D-maltopyranoside | DM | Yes | O | |
| n-Dodecyl-β-D-maltopyranoside | DDM | Yes | M | |
| n-Tetradecyl-β-D-maltopyranoside | TDM | Yes | O | |
| n-Dodecyl-β-D-maltopyranoside + Cholesteryl hemisuccinate | DDM/CHS | Yes | M | 21nM |
| n-Dodecyl-β-D-maltopyranoside + Cholesteryl hemisuccinate + 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate | DDM/CHS/CHAPS | Yes | M | 35nM |
| n-Octyl-β-D-glucopyranoside + Cholesteryl hemisuccinate | OG/CHS | No | N.D. | |
O: oligomer, M: monomer.
*
Multiple oligomeric states of CCR1 were observed to varying degrees in all of the detergent systems tested, but for the sake of clarity only the predominant state is shown in the table.