Figure 1.

DNA binding ability of Zur. The upstream region of znuA (panel a) or rovA (f), with or without a predicted Zur binding site, respectively, was amplified by PCR and used as target DNA probe in EMSA. For EMSA, the [γ-³²P]-labeled target DNA probes (1000 to 2000 c.p.m/μl) were incubated with the Zur protein in the presence or absence of 100 μM ZnCl₂. Increasing amounts of Zur (b and g), ZnCl₂(c), or EDTA (d and e) were employed. The mixtures were directly subjected to 4% polyacrylamide gel electrophoresis. The rovA gene was used as negative control.