Table 5.
Percentages of apoptotic and necrotic cells in cultures of IL-2-dependent primary (j, 43) and immortalised (line 5, S9) T lymphoblasts (0 h) and at different time points after IL-2 withdrawal (2 h, 8 h, 24 h), analysed by flow cytometry following Annexin-V staining.
| Cell lines | 0 h1 | 2 h | 8 h | 24 h | ||||
| A2 | N3 | A | N | A | N | A | N | |
| j | 0.24 | 2.1 | 0.9 | 1.1 | 0.4 | 2.2 | 0.5 | 2.0 |
| 43 | 0.5 | 3.0 | 0.7 | 2.0 | 0.4 | 2.2 | 0.7 | 3.0 |
| line 5 | 2.3 | 4.0 | 3.8 | 2.3 | 4.6 | 2.0 | 8.1 | 3.8 |
| S9 | 5.4 | 1.3 | 6.7 | 1.3 | 8.4 | 1.5 | 6.9 | 1.1 |
1hours after IL-2 withdrawal
2A – apoptosis
3N – necrosis
4percentage; mean of two replicates