(A), DU145 cells were treated with or without 50 μM DIM for 24 hours, after which total lysates were prepared and Western blotting conducted. Membranes were probed with indicated antibodies and visualized by ECL detection system. (B), DU145 cells were treated with or without 50 μM DIM and the p38 MAPK inhibitor SB202190 (SB) and subjected to Western blot analysis as in A. (C), DU145 cells transfected with or without dominant negative p38 MAPK (dnp38), were treated with 50 μM DIM and subjected to Western blot analysis as in A.