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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Cancer Res. 2009 Jun 23;69(13):5531–5536. doi: 10.1158/0008-5472.CAN-08-4834

Figure 2. An N-terminal region of BRCA2 and MCPH1 C-terminal BRCT domain are required for the MCPH1-BRCA2 interaction.

Figure 2

(A) Plasmids encoding seven GST-tagged fragments of BRCA2 were expressed in 293T cells with Myc-tagged MCPH1. GST pull-downs from 293T lysates were immunoblotted with anti-Myc and anti-GST antibodies. (B and C) Plasmids encoding FLAG-tagged fragments of B2F1 (residues 1−472) and FLAG-tagged deletion mutants of B2F12 (residues 234−335) were co-transfected with Myc-tagged MCPH1 into 293T cells. Lysates (WCL) were inmmunoprecipitated with FLAG-agarose beads and immunoblotted with anti-Myc and anti-FLAG antibodies. (D) Plasmids encoding Myc-tagged full-length and deletion mutants of MCPH1 were co-transfected into 293T cells with GST-tagged B2F1. GST pull-downs were immunoblotted with anti-GST and anti-Myc antibodies. Dotted lines represent deleted regions.