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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Cancer Res. 2009 Jun 23;69(13):5531–5536. doi: 10.1158/0008-5472.CAN-08-4834

Figure 4. The MCPH1-BRCA2 interaction influences homologous recombination repair.

Figure 4

(A) Diagram of the homologous recombination repair reporter assay. (B) Homologous recombination repair activity in a HeLa DR-GFP cell line is reduced relative to vector controls when MCPH1 is depleted by MCPH1 siRNA. (C) Homologous recombination repair activity in a HeLa DR-GFP cell line following co-transfection of I-Sce1 plasmid and BRCA2 deletion constructs with the MCPH1 interaction domain (B2F12, B2F12Δ5) and without the interaction domain (B2F12ΔR6−8). (D) Homologous recombination repair activity in a V-C8 DR-GFP cell line, using BRCA2-Δ328−351 and BRCA2-D2723H full-length mutant constructs.