Table 1. Kinetic analysis of GK and GKK169A.
Mutants | Protein conc. (mg/ml) | Vmax_Glucose (µM/min/mg) | S_Glucose 0.5 (mM) | Km_ATP (mM) | nH |
LGK2-WT | 2.24 | 3966.13±1107.69 | 11.64±0.83 | 0.30±0.01 | 1.40±0.12 |
K169A mutant | 2.24 | N/A | N/A | N/A | N/A |
Data are means±SEM for wild-type hLGK2 and hLGK2 mutants. The results are means of kinetic analyses of three independent experiments of wild-type hLGK2 and hLGK2 mutants. nH is the Hill number.