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. 2009 Jul 17;4(7):e6280. doi: 10.1371/journal.pone.0006280

Figure 3. Cellular translocation assay of F313X/F314X mutants.

Figure 3

(A) PA83 was titrated, mixed with a fixed concentration of LFN-DTA (100 pM), added to CHO-K1 cells, and incubated at 37°C for 4 hours. The level of protein synthesis was measured by detecting [3H] leucine incorporation and was expressed as fraction of that observed with PA-WT. Only WT, S1 (deletion of F313 and F314) and DNI (K397D, D425K) are shown. (B) EC50 for all F313X/F314X mutants as calculated from sigmoidal fits to cytotoxicity experiments.