Figure 3. Mechanism of tumor cell cycle arrest by ADSC-CM.

A. Capan-1 cells were cultured with or without ADSC-CMfor 48 hours. Proteins were then extracted and subjected to immunoblotting for the indicated proteins as described in Material and Methods. Results are representative of at least 3 independent experiments. B. Quantification of immunoblot densitometry in control cultures (black bars) or cultures treated with ADSC-CM (open bars). Values are means±S.E. of three separate experiments with ADSCs from different donors. *: p<0.05; **: p<0.01.