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. 2009 Jul 20;4(7):e6291. doi: 10.1371/journal.pone.0006291

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Bakke et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Histogram displaying the frequency of the consensus RBS at varying spacer lengths. Spacer length refers to the number of base pairs between the 3′ end of the RBS and the 5′ end of the start codon for each gene. The consensus RBS generally lies between 4 and 8 base pairs upstream of the start codon.