Table 4. Comparison of putative glycoside hydrolase start sites.
Annotation | DNA coordinates | Start codon | Length |
IMG | 69942..72866 (+) | ATG | 2925 bp |
RAST | 69912..72866 (+) | GTG | 2955 bp |
JCVI | 69882..72866 (+) | ATG | 2985 bp |
Examination of an individual gene displays tendencies of the annotation services. RAST identifies GTG as the start codon for the gene, while IMG and JCVI select two ATG codons at different locations. Predicted start codon affects gene length.