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. 2009 Jul 20;4(7):e6291. doi: 10.1371/journal.pone.0006291

Table 4. Comparison of putative glycoside hydrolase start sites.

Annotation DNA coordinates Start codon Length
IMG 69942..72866 (+) ATG 2925 bp
RAST 69912..72866 (+) GTG 2955 bp
JCVI 69882..72866 (+) ATG 2985 bp

Examination of an individual gene displays tendencies of the annotation services. RAST identifies GTG as the start codon for the gene, while IMG and JCVI select two ATG codons at different locations. Predicted start codon affects gene length.