The role of Nramp metal transporters in suppression of oxidative damage by mM manganese.
A) The indicated strains were grown in air without shaking in liquid SC medium lacking lysine and supplemented with the designated concentrations of MnCl2. Following 16 hours incubation, total cell growth was monitored as a function of absorbance at 600 nm. B) The indicated strains were grown as above in (A), except medium was supplemented with lysine and 3 mM MnCl2. Total cellular manganese was determined by AAS as described in Materials and Methods. Values represent the average of three independent cultures with error bars representing standard deviation. Strains employed sod1 Δ, KS107; sod1 Δ smf1 Δ, SL109; sod1 Δ smf2 Δ, MC123.