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. Author manuscript; available in PMC: 2010 Jan 15.
Published in final edited form as: Free Radic Biol Med. 2008 Oct 14;46(2):154–162. doi: 10.1016/j.freeradbiomed.2008.09.032

Fig. 6.

Fig. 6

Studies with vtc4 Δ show a role for intracellular manganese but not phosphate in suppression of oxidative damage.

A) 105 and 104 cells of the indicated strains were spotted onto YPD plates supplemented where indicated with 5 mM MnCl2. Cells were allowed to grow for three days. B) Strains were grown in SC complete medium supplemented where indicated with the designated levels of MnCl2 prior to analysis of total cellular manganese (left) and iron (right) by AAS. In the absence of added manganese, the total manganese accumulation in the sod1 Δ and sod1 Δ vtc4 Δ mutants is 2.54 and 2.25 nmoles/109 cells respectively. C) Orthophosphate analysis of the indicated strains grown in SC medium was carried out as in Fig. 5B. D) Test for lysine independent growth as in Fig. 2A was conducted in the designated strains supplemented with the indicated levels of MnCl2. Strains employed include: WT, BY4741; vtc4 Δ and vph1 Δ, the corresponding kanMX4 derivates of BY4741; sod1 Δ, LJ284; sod1 Δ vtc4 Δ, LJ286; sod1 Δ vtc4 Δ pmr1 Δ, LJ285; sod1 Δ pmr1 Δ, LJ283. (B–D) results represent the averages of 3–4 independent cultures and error bars represent standard deviation.