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. Author manuscript; available in PMC: 2009 Jul 8.
Published in final edited form as: J Pineal Res. 2008 Oct 28;46(2):161–171. doi: 10.1111/j.1600-079X.2008.00644.x

Fig. 3.

Fig. 3

Pretreatment of MT2-CHO cells with melatonin (400 pM for 8 hr) followed by a 16 hr withdrawal of melatonin (M/V) resulted in a loss of high potency sites (-high) and a shift to low potency states (-low) when compared to vehicle (V/V)-exposed cells. However, Colcemid, added during the 16 hr withdrawal period (M/C), prevented the loss in the number of high-potency states when compared to melatonin-treated cells. The addition of Colcemid during the 8 hr pretreatment period (data not shown) or during the 16 hr withdrawal period (V/C) yielded effects similar to vehicle -treated cells. Data are representative of 3–7 experiments performed in triplicate or quadruplicate. Data points were fit by 1-site or 2-site fit non-linear regression analysis (GraphPad Prism, San Diego, CA) based on residual sum of squares. Potency (IC50) values shown above the graphs represent the mean and range of SEM of 3–5 experiments performed in duplicate.