Skip to main content
. Author manuscript; available in PMC: 2010 Mar 6.
Published in final edited form as: Cell. 2009 Feb 26;136(5):939–951. doi: 10.1016/j.cell.2009.01.025

Figure 2.

Figure 2

pptr-1 regulates lifespan, thermotolerance and fat-storage through the IIS pathway. Data shown are from one representative experiment.

A) pptr-1 RNAi significantly reduces the lifespan of daf-2(e1370) mutants similar to daf-18 RNAi (mean life on vector RNAi is 33.9 ± 0.7 days (n=77), pptr-1 RNAi is 27.7 ± 0.9 days (n=63) p <0.0001and on daf-18 RNAi is 20.4 ± 0.6 days (n=40), p <0.0001)

B) pptr-1 RNAi does not affect the lifespan of wild-type worms (mean lifespan on vector RNAi is 22.8 ± 0.4 days (n=61), pptr-1 RNAi is 21.9 ± 0.5 days (n=49). daf-18 RNAi reduces mean lifespan of wild type worms to 18.6 ± 0.3 days (n=48) p <0.0001).

C) The thermotolerance of daf-2(e1370) worms is reduced by pptr-1 as well as daf-18 RNAi (mean survival of daf-2(e1370) worms at 37 °C on vector RNAi was 15.2 ± 0.7 hrs (n=34), whereas on pptr-1 RNAi the survival was 13.8 ± 0.5 hrs (p value< 0.006) (n=36) and 10.3 ± 0.7 hrs (p value< 0.0001) (n=29) on daf-18 RNAi. pptr-1 RNAi did not affect the thermotolerance of wild type worms; (mean survival was 9.8 ± 0.4 hrs on vector RNAi (n=32), 9.3 ± 0.3 hrs on pptr-1 RNAi (n=35) and 9.7 ± 0.4 hrs on daf-18 RNAi (n=32).

D) Sudan black staining showing that pptr-1 RNAi reduces the increased fat storage of daf-2(e1370) worms, similar to daf-18 RNAi but has no effect on wild type fat-storage. Arrows indicate the pharynx. A representative picture from one of three independent experiments (n=30) is shown.