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. Author manuscript; available in PMC: 2009 Jul 8.
Published in final edited form as: Biochemistry. 2008 Apr 11;47(18):5194–5204. doi: 10.1021/bi800292v

FIGURE 1.

FIGURE 1

Reconstitution of cholesterol-transfer activity using mouse liver CMVs. CMVs were prepared from the livers of G5G8−/− mice and from mice infected with adenoviruses that either express G5 and G8 or have no insert. Inside-out vesicles were made from the CMV- and ATP-dependent [3H]-cholesterol-transfer assays and were performed as described in the Experimental Procedures. (Inset) Immunblot analysis of G5 in the whole liver homogenate (25 µg), the postnuclear fraction (25 µg), and the CMVs (5 µg) of mice expressing G5 and G8 using a rabbit polyclonal antihuman G5 Ab (5). A total of 25 µg of protein from each fraction was subjected to immunoblotting using an anti-LDLR Ab (39). Alkaline phosphatase activity was measured as described in the Experimental Procedures using 25 µg of membrane protein.