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. Author manuscript; available in PMC: 2009 Jul 8.
Published in final edited form as: Biochemistry. 2008 Apr 11;47(18):5194–5204. doi: 10.1021/bi800292v

FIGURE 3.

FIGURE 3

Chemical cross-linking of the G5G8 complex. Partially purified native G5G8 (20 µg) that was obtained after fractionation over a Blue 2 column (see the Experimental Procedures) was incubated at room temperature for 15 min in the presence or absence of ATP (5 mM), MgCl2 (2 mM), NaVO4(1 mM), and BeFx (1 mM/5 mM). The proteins were then chemically cross-linked using disuccinimidyl glutarate (DSG) according to the instructions of the manufacturer. The proteins were then analyzed by SDS–PAGE and Western blotting.