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Purification of native mouse G5G8 by G5 Ab-affinity chromatography. Native G5G8 was affinity-purified using G5 Ab-conjugated beads as described in the Experimental Procedures. An aliquot of the purified protein (25 ng) was treated with peptide N-glycosidase F (PNGase), and the proteins were size-fractionated on a 12.5% SDS–PAGE before the gel was stained with silver (top) or analyzed by Western blotting (0.5 ng of protein) using anti-G5 and anti-G8 antibodies (bottom).
