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. Author manuscript; available in PMC: 2009 Jul 8.
Published in final edited form as: Biochemistry. 2008 Apr 11;47(18):5194–5204. doi: 10.1021/bi800292v

FIGURE 6.

FIGURE 6

Protein concentration dependence and time course of cholesterol-transfer activity by purified native mouse G5G8. (A) Purified native G5G8 prepared as described in the Experimental Procedures was concentrated using a Microcon filter (Amicon) and reconstituted into proteoliposomes. In this assay, the concentration of G5G8 was such that the same volume of proteoliposomes was used for each cholesterol uptake assay. ATP-dependent cholesterol transfer was measured as described in Figure 5. (B) Time course of ATP-dependent cholesterol uptake was determined by measuring ATP-dependent [3H]-cholesterol transfer from the donor liposomes to the proteoliposomes containing native G5G8 (20 ng) at the designated time intervals.