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Functional analysis of the palmitoylation of G5. G5G8−/− mice were infected with adenoviruses encoding wild-type or mutant G5 (C61A) and wild-type G8 (8 mice/group). (A) Immunoblot analysis of liver membrane fractions from a representative mouse from each group was performed using antibodies to mouse G5 (4591) and G8 (1B10A5). (B) Gallbladder bile was collected following a 4 h fast, and biliary cholesterol was measured using GC–MS, as described in the Experimental Procedures.
