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. 2009 May 5;284(28):18715–18725. doi: 10.1074/jbc.M109.015099

FIGURE 2.

FIGURE 2.

K+ dependence of Na+,K+-ATPase activity. Measurements were performed at 37 °C in 30 mm histidine (pH 7.4), 40 mm NaCl, 3 mm ATP, 3 mm MgCl2, 1 mm EGTA, 10 μm ouabain, and the indicated concentrations of K+ added as KCl. Filled circle, wild type; open triangle pointing upward, ΔYY; filled triangle pointing upward, ΔTYY; dotted triangle pointing upward, ΔWVEKETYY; open triangle pointing downward, Y1018F; filled triangle pointing downward, Y1018A; dotted triangle pointing downward, YY-AA; open diamond, R936A; crossed diamond, R935A; open square, EKE-AAA; filled square, K768M; crossed square, K768A. For comparison, the wild type is shown in all panels. The K0.5(K+) values for the activating phase are indicated in Table 1.