FIGURE 4.

AG1024 increases the aggregation of extracellular ADDLs. Cultured rat primary cortical neurons were treated with ADDLs, insulin and IGF-1 with or without preincubation with AG1024. In addition, a negative IGF-1R antibody was added to neurons for 15 min to inhibit the IGF-1R activity. A, the extracellular medium from each condition was concentrated. B, the cell lysates from the same conditions were immunoprecipitated (IP) with the 6E10 antibody. C, NIH3T3 cells with or without expression of IR or IGF-1R treated in similar conditions and the medium processed as in A. All samples were resolved on SDS-PAGE. The species of ADDLs were then assessed on Western blots with 6E10.