FIGURE 3.

Activation of the ROS-PI3K pathway by CoCl₂ and intermittent hypoxia in PC12 cells. A, expression of reporter activity induced by sustained hypoxia in PC12 cells. B, inhibition of CoCl₂-induced luciferase activity by NAC. C, LY294002 (50 μm) inhibited CoCl₂-induced HIF-1α activity (left panel) and the induction of IPAS mRNA in RPMI (right panel). D, TH and IPAS gene expression in PC12 cells exposed to IH in different culture media. 3Mix indicates a mixture of magnesium, His, and Thr as mentioned in Fig. 1C. E, expression of reporter activity in PC12 cells exposed to IH in different culture media. F, inhibition of reporter activity by NAC in PC12 cells exposed to IH. *, p < 0.05 for indicated comparison. **, p < 0.01 for indicated comparison. The data shown in the bar graphs are the averages ± S.D. of three independent experiments.