FIGURE 5.

Hypomagnesemia suppresses response to CoCl₂ in the CB. A, in situ hybridization of TH expression in the CB of CoCl₂-injected magnesium-deficient mice. The mice were dissected at 24 h after CoCl₂ administration. The sections were counterstained with kernechtrot. CBs with higher magnification images are also shown (rectangle area). The cells were divided into four groups (i = 0–3) in terms of TH expression levels as shown in Fig. 4C, and cells with respective expression levels were scored. TH mRNA was constitutively expressed in neurons of the superior cervical ganglion regardless of CoCl₂ treatment. B, immunohistochemical analysis of TH expression in the CB of CoCl₂-injected magnesium-deficient mice. The cells were divided into four groups as shown in Fig. 4C. The data shown in the bar graphs are the averages ± S.D. of three independent experiments.