FIGURE 4.

Iron mineralization kinetics in the presence of L. innocua Dps and its ferritin-like pores mutants. Fe(II) oxidation was monitored in air at 350 nm after mixing a 2.0 μm protein solution in 100.0 mm MOPS buffer, pH 7.0, with a small volumes of FeSO₄ solution in 1.0 mm HCl. The kinetic curves presented are representative of three to five experiments with two or three independent protein preparations (see Table 1 for the experimental variability). LiDps (solid line), D130N (dashed line), and D121N/D126N/D130N (dotted line). The D121N and D126N curves are superimposable to that of LiDps and have been omitted from the figure.