FIGURE 3.

NFAT-3 is nuclear and occupies the GAP-43 promoter in the developing mouse cortex. A, NFAT-3 is localized in the nucleus of cortical neurons. Fixed mouse P1 brain was probed for immunohistochemistry with α-NFAT-3, α-GAP-43, and DAPI nuclear staining. Scale bar: 30 μm. B, NFAT-3 occupies the GAP-43 promoter in the mouse brain. ChIP was performed for NFAT-3 using the same antibody as in Fig. 2, from fixed P1 mouse brain, and recovered DNA was measured by semiquantitative PCR. GAP-43-specific primers spanning the putative NFAT site were used. The resulting PCR amplicon containing the NFAT site of the GAP-43 promoter is depicted. No Ab, no antibody.