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Analytical ultracentrifugation sedimentation velocity ls-g(s) versus s20,w curves computed with SEDFIT. A, Hsp27-wt at 0.66 mg/ml (red), 0.22 mg/ml (blue), and 0.03 mg/ml (green). B, Hsp27-3D at 0.53 mg/ml (red), 0.32 mg/ml (blue), 0.09 mg/ml (green), and 0.03 mg/ml (black). C, Hsp27-3P at 0.47 mg/ml (red), 0.22 mg/ml (blue), and 0.02 mg/ml (green) at 37 °C in 120 mm NaCl, 25 mm Tris, 2 mm MgCl2, 0.1 mm EGTA, 0.01% NaN3, 50 μm PMSF, 5 mm DTT, pH 7.5.
