TABLE 1.
Summary of the IAANS fluorescence results of HCM cTnC mutants
The pCa50 and nHill were calculated for both classes of sites from independent Ca2+ titrations, and error is reported as ±S.E.
TnC | High affinity sites |
Low affinity site |
||
---|---|---|---|---|
pCa50 | nHill | pCa50 | nHill | |
WT | 7.03 ± 0.01 | 1.85 ± 0.02 | 4.91 ± 0.01 | 0.90 ± 0.01 |
A8V | 6.90 ± 0.01a | 1.56 ± 0.05a | 4.89 ± 0.03 | 0.91 ± 0.02 |
E134D | 6.91 ± 0.01a | 1.90 ± 0.06 | 4.92 ± 0.02 | 0.94 ± 0.03 |
D145E | NDb | ND | 5.02 ± 0.02a | 0.88 ± 0.01 |
WT-C84S | 7.06 ± 0.01 | 0.94 ± 0.03 | 4.44 ± 0.01 | 1.15 ± 0.04 |
C84Y | 6.92 ± 0.01a | 1.02 ± 0.02 | 4.43 ± 0.02 | 1.42 ± 0.06 |
ap < 0.05 compared with their respective WT.
b ND, not determined.