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. 2009 May 12;284(28):19090–19100. doi: 10.1074/jbc.M109.007021

TABLE 1.

Summary of the IAANS fluorescence results of HCM cTnC mutants

The pCa50 and nHill were calculated for both classes of sites from independent Ca2+ titrations, and error is reported as ±S.E.

TnC High affinity sites
Low affinity site
pCa50 nHill pCa50 nHill
WT 7.03 ± 0.01 1.85 ± 0.02 4.91 ± 0.01 0.90 ± 0.01
A8V 6.90 ± 0.01a 1.56 ± 0.05a 4.89 ± 0.03 0.91 ± 0.02
E134D 6.91 ± 0.01a 1.90 ± 0.06 4.92 ± 0.02 0.94 ± 0.03
D145E NDb ND 5.02 ± 0.02a 0.88 ± 0.01
WT-C84S 7.06 ± 0.01 0.94 ± 0.03 4.44 ± 0.01 1.15 ± 0.04
C84Y 6.92 ± 0.01a 1.02 ± 0.02 4.43 ± 0.02 1.42 ± 0.06

ap < 0.05 compared with their respective WT.

b ND, not determined.

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