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. 2009 May 12;284(28):19090–19100. doi: 10.1074/jbc.M109.007021

TABLE 2.

Summary of pCa50 of Ca2+ affinity or Ca2+ sensitivity measured by various experimental assays at different levels of complexity of the thin filament

TnC pCa50
cTn TF ATPase 100% ATPase 50%a Skinned fiberb
WT 6.69 ± 0.01 6.13 ± 0.02 5.69 ± 0.05 5.68 ± 0.02 5.66 ± 0.01
A8V 6.58 ± 0.01c 6.27 ± 0.02c 6.20 ± 0.03c 5.87 ± 0.03c 6.02 ± 0.01c
E134D 6.69 ± 0.01 6.11 ± 0.02 5.72 ± 0.03 5.66 ± 0.04 5.68 ± 0.01
D145E 6.81 ± 0.01c 6.21 ± 0.02c 5.94 ± 0.03c 5.90 ± 0.02c 5.90 ± 0.01c
C84S 6.77 ± 0.01 NDd ND ND ND
C84Y 6.76 ± 0.01 ND 6.25 ± 0.03c 6.20 ± 0.03 5.93 ± 0.01c

a WT in ATPase 50% was measured at 100% ratio and compared with the mutants at a 50% ratio.

b Skinned fiber values were obtained from Ref. (9).

cp < 0.05 compared with their respective WT.

d ND, not determined.

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