TABLE 3.
Summary of circular dichroism results for HCM cTnC mutants
The CD spectrum is reported for apo (without divalent cations), Mg2+-bound, and Ca2+/Mg2+-bound states, and error is reported as ± S.E. deg, degrees.
TnC | [θ]222 nm |
n | ||
---|---|---|---|---|
Apo | Mg2+ | Ca2+/Mg2+ | ||
deg·cm2/dmol | ||||
WT | −16,178.9 ± 178.9 | −17,945.4 ± 327.5 | −20,124.2 ± 212.2 | 3 |
A8V | −17,276.2 ± 168.0a | −19,952.6 ± 37.2a | −21,848.0 ± 199.2a | 3 |
C84Y | −14,786.7 ± 73.6a | −17,892.0 ± 136.9 | −20,545.5 ± 98.7 | 3 |
E134D | −16,027.7 ± 177.5 | −18,077.7 ± 233.9 | −20,788.3 ± 196.0 | 3 |
D145E | −15,246.2 ± 105.9a | −17,203.6 ± 202.5 | −19,064.7 ± 169.5a | 3 |
a p < 0.05 compared with the WT at same condition.